Effect Combination of Catharanthus roseus and Phyllanthus urinaria Extract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression

CD4+CD25+FOXP3+ is a regulatory T cell that plays role in the body's tolerant system to control activated effector cells. an important as trigger for tumor progressiveness, where increase amount of FOXP3 state correlates with poor prognosis. Breast cancer type tends every year. The purpose this study was influence combination Phyllanthus urinaria and Catharanthus roseus plant extracts on modulation. Mice injected subcutaneously carcinogen compound DMBA six weeks at 0.015 mg/kg weight. design conducted using complete random (RAL) which divided into groups namely healthy (normal) control, (DMBA injection), Cisplatin three different doses (dose 1 (Phyllanthus 500 weight 75mg/kg weight) dose 3 urinary 2000mg/kg 375mg/kg weight). relative number cells measured flow cytometry analysis. measurements were made Cell Quest ProTM program. Statistics analyze one way ANOVA (p<0.05). Tukey HSD analysis SPSS 26 software used real differences between treatments. A occurs control. While decrease occurred treatment except group 1. optimal reduce amounts significantly 3. This indicates differentiation. Keywords: breast cancer, roseus, CD4+CD25+FOXP3+, urinaria,